Dissolved oxygen levels in liquids are not necessarily the same as in the surrounding atmosphere. When a cell culture medium is taken from ambient air into a hypoxia workstation, it can take a surprisingly long time for its dissolved gases to reach equilibrium with the surrounding modified atmosphere. Now, researchers have an alternative to leaving media to condition for 24 hours.
With the Whitley Media Conditioner (UK Patent No. GB2579704) you can equilibrate your liquid media rapidly and ensure even greater accuracy in your research. Although this product was designed to be used inside the Hypoxystation range of workstations, microbiologists may benefit from a device of this type when reduced atmospheric oxygen concentrations are of interest.
Although Whitley Workstations allow tight control of the conditions under which cells/bacteria are cultured, and avoid even very brief exposures to elevated oxygen concentrations, cells may still be unintentionally exposed to excess oxygen during media changes if the fresh media has not previously been equilibrated with the incubation atmosphere.
- Equilibrate up to 400ml of liquid medium with an atmosphere containing 0.1% oxygen in just 65 minutes.
- Equilibration of smaller volumes (100ml or 200ml) in atmospheres containing higher oxygen concentrations requires as few as 12 minutes.
- Cycles optimised for use at a temperature of 37°C.
- The unit fits perfectly into the airlock of a Whitley Hypoxystation so can be transferred easily into the chamber environment for use and then removed when necessary.
- A fast, flexible system that will save a considerable amount of time and is a new tool for improving outcomes in your research.
- Magnetic stirrer
- 2 x Sealing slips
- 2 x 500ml Duran bottles
- 24V Power supply
- Mains lead
- User guide
|Oxygen Conc. (%)||Volume / 100 ml||Volume / 200 ml||Volume / 400 ml|
||40 minutes||60 minutes||95 minutes|
|0.1 - 1.0||30 minutes||40 minutes||65 minutes|
|3||14 minutes||20 minutes||35 minutes|
|5||12 minutes||15 minutes||25 minutes|