Gradient Chip

The Physiochip family comprises several microfluidic based culture chambers which aim to create cell and tissue culture environments that resemble the in-vivo conditions.

Gradient Chip

The Gradient Chip is an easy-to-use cell culture system that permits to generate long-term stable gradient profiles of soluble molecules and dissolved oxygen in 20 and 3D environments. The chip is compatible with adherent and non-adherent cells, which can be grown on the bottom of the chamber or embedded in gel matrices. Live microscopy imaging of cell behavior is straightforward thanks to its optical grade bottom.

Features:

  • Long-term stable gradients of soluble molecules and dissolved oxygen
  • 2D and 3D environments
  • Coverslip-like bottom compatible with high-end microscopy
  • Small volume - minimal amount of medium and supplement
  • Optimized chip holder for easy manipulation
  • Fully integrated solution - pumping systems and accessories available
 

Gradients in cell culture systems

Biomolecule gradients have been shown to play essential roles in a wide range of biological processes including development, inflammation, cell migration, wound healing, and cancer. Elucidation of these phenomena requires the ability to expose cells to biomolecule gradients that are quantifiable, controllable, and mimic those that are present in vivo.

Optimal shear stress-free gradient conditions for Imaging

Inside the Gradient Chip, cells are subject to a shear stress-free environment and culture media can be replaced continuously, what turns into an excellent control of the gradient profile during periods of > 1 week with the possibility of instantly altering the gradient shape during the course of the experiment. The system is fully compatible with standard inverted microscopes and in vivo cell imaging equipment.

How it works

Cells are injected and seeded on the bottom of the chip or embedded in a 3D gel. The gradient is generated by diffusion of the factor of interest (e.g. a soluble molecule or dissolved oxygen) between two lateral channels through the culture volume/area. The chip is encapsulated in a holder that facilitates its manipulation and operation and provides easy connection of tubing to access ports. The only required additional equipment is a pumping system. Different models are available in EBERS' catalogue, although every member of PhysioChip family is compatible with most commercial peristaltic and syringe pumps.

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Download 2D Instructions for Use

Download 3D Instructions for Use

Gradient Chip

MC3T3 cells after 24 hours of culture in a collagen gel under a gradient of dissolved oxygen in the Gradient Chip system (Cout1esy of I. Ochoa, 13A, Spain)

Gradient Chip

Applications:

  • Chemotaxis of adherent and non-adherent cells
  • Tumor microenvironment
  • Migration assays
  • Angiogenic sprouting
  • Modulation of cell behavior by oxygen concentration (differentiation, cancer, metastasis…)
  • Axon guidance

Technical Information:

  • Outer dimensions of the holder: 63 mm x 30.5 mm (diameter x height)
  • Thickness of the culture chamber: 300 um
  • Growth surface area of the chamber (2D configuration): 8 mm2
  • Volume of the chamber (3D configuration): 2.4 ul
  • Bottom thickness: 100 um